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Railroad giant CSX, which is under fire from regulators for operating changes that have disrupted its freight service nationwide, laid off more than 6o employees this week at its primary Chicago switching yard, according to the workers’ union.

Truckload carrier U.S. Xpress Enterprises said it is laying off 100 employees — all nondrivers — at two southeastern locations, citing a change in “company focus.”

Comprehensive Logistics will lay off employees in January, according to a Worker Adjustment and Retraining Notice Act document filed with the Ohio Department of Job and Family Services.